The 31-year-old has won 18 medals at junior and senior track world championships, the Commonwealth Games and Olympics, forming a world-leading partnership with Eddie Dawkins and Ethan Mitchell.
Over the four years from 2014, the trio formed the pre-eminent team sprint combination on the globe — Dawkins retired in 2021 and Mitchell earlier this year.
Webster came into prominence when he won the sprint, team sprint and keirin gold medals at the junior world championships in Moscow in 2009, and two medals followed as a teenager at the 2010 Delhi Commonwealth Games.
The New Zealand trio first made their mark with a bronze in the team sprint at the 2012 world championships and in 2014 won gold in the team sprint in Cali, Colombia.
Over the four years from 2014, they won three rainbow jerseys plus a silver. They followed with silver at the Rio Olympics, pipped by Great Britain for the gold.
"I have given the sport absolutely everything, every day at training and every time I went on to the track anywhere in the world.
‘‘And have been rewarded with so much enjoyment and so many memories," Webster said.
"I want to thank my coaches throughout my career, the staff at Cycling New Zealand and HPSNZ, Ethan, Eddie and my other team-mates both past and present, the fans, our commissaires and volunteers — everyone who has helped me on my journey.
"I know it is time and I leave with absolutely no regrets. I do not know what is ahead.
‘‘I will take some time to decompress, but I am really excited about exploring what future opportunities may arise in life after competition."