The six people, who included a man in his 20s and another man in his 30s, helped on the relay in the southern prefecture of Kagoshima on April 27, Tokyo 2020 said in a statement late on Saturday.
All of them assisted with traffic control and wore masks, with three of them doing so in the city of Amami and the other three in the city of Kirishima, said the organisers.
Earlier last month, two other people who helped with the torch relay on the southern island of Shikoku were diagnosed with Covid-19, including a police officer in his 30s who guided traffic in Kagawa prefecture.
The Tokyo 2020 organisers urged people to comply with Covid-19 guidelines.
Japanese authorities are determined to protect Games participants and a local population that opinion surveys have shown is largely opposed to the Olympics due to fears the event will lead to more Covid-19 infections.
Japan last month declared a Covid-19 state of emergency for the major population centres of Tokyo and Osaka and two other areas set to last until May 11 to contain a fourth wave of infections.
The Games, delayed by a year because of the pandemic, are scheduled to open on July 23.
