A St John spokeswoman said one patient was taken to Dunedin Hospital after the two-car crash in South Rd about 1.05pm.
The spokeswoman said another patient, in a minor condition, was treated at the scene.
A Fire and Emergency New Zealand spokesman said a rescue tender from Lookout Point Station attended the incident along with a crew from St Kilda.
Nobody was trapped.
Crews assisted police and providing first aid at the scene until the ambulance arrived, the spokesman said.
A police spokesman said the crash occurred when a car hit a tree at the intersection of Portobello Rd and Doon St about 3.40pm.
A St John spokeswoman said one ambulance attended and one person in a moderate condition was taken to Dunedin Hospital.
A Fire and Emergency New Zealand spokeswoman said two crews, from Lookout Point and St Kilda, also assisted.
