It will face Southland tomorrow in two warm-up games at Ellis Park, as it looks to prepare for December's Jubilee Cup.
Having been re-established last year after a seven-year hiatus, it has been nine years since the side hosted another province in Dunedin.
That is exciting for the team, although coach Marg Daniels said it remained very much in warm-up mode.
Winter skills training sessions - held for all women in Otago - had proved beneficial and those who attended stood out at trials.
Now playing together in a game and adapting to the women's game were the focuses, both tomorrow and in two weeks in Invercargill.
``It's different when you play women against women, compared to men against women,'' Daniels said.
``They field differently and that's what I've got to try to get through to the team now.
``Because on a Saturday they go out there and you see them just about at the outfield fence because of the way the men bat.
``Whereas when they're playing women they don't need to do that, so they've got to be remembering all the time to be coming in.
``It's quite a different game.
``The same in the batting box - they tend to stand in the back of the batting box when they're facing a man pitcher.
``Whereas this is going to be good facing some women prior to going to Jubilee.
``They'll be able to come up and meet that ball in fair territory, so it should make a difference.''
Daniels felt Otago had a tidy fielding team and some players who could hit the ball well.
While it would be tough against Canterbury, she felt it should hold up well against Southland.
The basis of the squad was the same as the resurrected one selected by Sandy Wallace last year, although there were some new faces.
Daniels said there were plenty of players who could be key among the team.
Pitcher Stacey Wardell had put in plenty of work over winter and had made big improvements, while youngster Kaitlyn Hastie would also feature in the team.
The team was missing Samara Henry, who had an ankle injury, and had been replaced by Jackie Padman.