The airline's first flight is set to land next Tuesday and 17 staff from Dunedin have been employed for the service.
Pacific Blue spokesman Phil Boeyen said the permanent part-time staff would provide ticketing and boarding services and would also load bags and clean the interior of the plane.
The staff would be employed for about three hours a day, and it was logical to employ people from Dunedin rather than fly people from Christchurch.
The Pacific Blue daily service would arrive at 12.15pm from Christchurch and return to Christchurch at 1pm.
The Boeing 737-800, which is part of the existing fleet, carries 180 passengers and will be full when it arrives at the airport on Tuesday.
Only a few seats remained for the Christchurch return flight.
Meals or snacks would not be served.
Demand was strong for the service, despite tougher economic times.
Mr Boeyen could not supply information on how many tickets had been sold for Dunedin flights.
There were no plans at this stage to have a connection between Dunedin and transtasman destinations, nor was there any proposal for Pacific Blue to fly into Queenstown.
On board the inaugural flight will be Pacific Blue chief executive John Barnett and Dunedin Mayor Peter Chin.
A ceremony will be held after the plane lands.
