After the shock dumping of replacement Sam Moran in January, original Yellow Wiggle Greg Page returned to the stage, only to announce on Thursday that he'll be passing on the well-worn top in December to Emma Watkins - the first female member of the group.
Watkins is not new to the mega-earning Wiggles - for the past two years the 22-year-old has taken on the roles of Fairy Larissa, Dorothy the Dinosaur, Wags the Dog and a Wiggly Dancer.
Jeff Fatt and Murray Cook will also pass on their famous purple and red tops to a new generation of performers, when The Wiggles wind up their Celebration world tour in Australia in December.
Blue Wiggle Anthony Field, 49, will continue driving in the Big Red Car.
"The touring and performing over the past 21 years has meant that we've spent a long time away from our own families and friends," Cook said in a statement.
"We miss them and want to spend more time at home which is a major reason why three of us decided it was time to hand on our skivvies to a new generation."
Newcomer Watkins will be joined by Lachlan Gillespie and Simon Pryce who will become the new Purple and Red Wiggles.
"We've been entertaining children around the world for 21 years and it's important that we plan for the future so that The Wiggles can keep wiggling in the years to come," Cook added.
Page, 40, had only recently rejoined the entertainers after leaving in 2006 due to a mystery illness, later diagnosed as the debilitating disease dysautonomia.
"It's been so great having Greg back with us so far this year, and to finish our time on stage all together again seems so fitting," Fatt said.
Page came back at the request of the other three Wiggles, after they got together to be inducted into the ARIA Hall Of Fame in 2011.
Although Moran had agreed to step aside for the reunion, fans were outraged at the sudden announcement.
Moran, who seemed bemused at the time at the attention he was getting, said: "All the support I've received from families and fans online ... has been incredible and really humbling for me."
Page is not the only Wiggle to have had health problems, with 58-year-old Fatt undergoing heart surgery in July 2011.
Added to a gruelling tour schedule, this caused the group to cancel an appearance at Sydney's Carols in the Domain last year.
On Thursday Page, Fatt and Cook, 51, thanked their fans for 21 fantastic years in the Big Red Car. From 2013 the trio will take on backstage, creative roles.
