Prop Beth Mallard, utility back Kelly Brazier and outside back Carla Hohepa have all been named for the Black Ferns side to tour England next month.
All three are aiming for the World Cup in England next year.
Former Otago inside back Barbara Chittock, who now captains Canterbury, has also been named in the 26-strong team for the first time.
Brazier is also a new cap, after a broken collar bone put paid to her chances of making the side last year.
Brazier (19), a first five-eighth or fullback, and said she had no real preference between the two positions, but just wanted to get involved.
"I'd probably prefer to know where I'm going to play as I can prepare for it. But I'll play anywhere, as long as I can get my hands on the ball," Brazier said.
She started off playing fullback this season before being switched to the No 10 jersey with some success in the final two games for Otago. She is also a handy goalkicker.
A second-year Sports Institute student, the former Otago Girls High School pupil now has to quickly finish assignments before leaving for a camp in Auckland in early November, and then flying out to England.
Hohepa (24) is another player who is looking at two positions.
She started off on the wing but was moved to centre in the last couple of games for Otago and said she wanted to play there as she got more ball.
She felt her season started a bit slowly but had improved as she got more time with the ball, and got rid of niggling injuries.
Hohepa, a primary school relief teacher and partner of Otago winger Karne Hesketh, said a tour to England was a great way to end the year, and would be great preparation for the World Cup next year.
Mallard (28), a tighthead prop, said the trip was fantastic for women players in New Zealand.
The Otago captain had a mixed provincial season, not helped by the side failing to make the semifinals.
"It's always hard to judge how you are playing when the team is not winning. You're not really going to be happy with your own game if you are coming off the field after a loss," she said.
Mallard, who is completing a PhD in physiology, studying liver immunisation at the University of Otago, is aiming for the World Cup. She said the two tests against England would be hard, as the home team would be very competitive.
The second test will be at Twickenham, just an hour after the All Blacks play England at the same ground.
"I've been to the ground watching and it is an amazing place. Hopefully, a few of the people will stay and watch after the All Blacks game."
The players may be sharing the ground with their male counterparts but financial compensation is miles apart.
The women will get a travelling allowance when on tour, but Mallard was not complaining, saying everything was paid for and getting this sort of trip was rare in women's sport.
• BLACK FERNS - Squad to tour England
Kelly Brazier, Carla Hohepa, Beth Mallard (Otago), Olivia Coady, Kendra Cocksedge, Barbara Chittock, Amanda Murphy, Casey Robertson, Amiria Rule, Melissa Ruscoe, Stephanie TeOhaere-Fox, Anika Tiplady, Kimberly Smith, Renee Wickcliffe (Canterbury), Fiao'o Faamausili, Victoria Grant, Victoria Heighway, Linda Itunu, Emma Jensen, Justine Lavea, Huriana Manuel, Karina Penetito, Vita Robinson (Auckland), Ruth McKay (Manawatu), Rebecca Mahony, Claire Rowat (Wellington).
