The union has also called an emergency meeting of club officials on Monday night to come up with an action plan on game day protocols.
The new directives are a reaction to a brawl in a senior match between Kurow and Athletic on May 9.
That resulted in three players being suspended: one for 13 weeks for biting, one for six weeks for fighting and another for three weeks, also for fighting.
North Otago Rugby Football Union president David Douglas said the union would be applying a "clean up policy" to any form of foul play or abuse.
"We need to respect each other and we need to respect the game of rugby. What happened at Kurow must never happen again," Douglas said.
Of concern to the union was not only the brawl and subsequent suspensions, but also allegations of verbal and racial abuse from spectators.
Douglas said he wanted rugby to be attractive to the next generation, and collaboratively "we must clean up our act".
Union chief executive Colin Jackson said the union wanted to show some action and make it clear it would not tolerate foul play.
The union would not name the three players suspended, but all were believed to be from Athletic.
Jackson said the referee and assistant referees will be asked to show zero tolerance of all forms of foul play and verbal abuse, on and off the field.
From May 30, all home teams in the six-team senior competition must video their games. The North Otago union will be giving $300 to clubs to cover costs.
The union said all clubs needed to address on and off-field issues with their own members, and set standards that reflected honesty, integrity and respect.
Jackson said the union never wanted to see the incident that occurred in Kurow happen again.
