Bolt stretched his arms out wide and put his palms out, then slapped his chest just before crossing the line. He came in well below his old mark of 9.72 seconds, set May 31 in New York - less than a year after Bolt took up the dash.
What had been billed as a showdown was not close at all. Richard Thompson of Trinidad and Tobago took the silver medal in 9.89, and Walter Dix of the United States was third in 9.91.
The 21-year-old Bolt's performance left no doubt whatsoever about who deserves the title of "World's Fastest Man."
Former world record-holder Asafa Powell of Jamaica was fifth, while world champion Tyson Gay of the United States failed to qualify for the final.
Bolt's specialty has been the 200, which he will be a heavy favourite to win next week in what would be first men's Olympic sprint double since Carl Lewis in 1988.