Defenders Hannah Broederlow, Jess Moulds and Jaimee Power and midcourters Camilla Lees and Sam Durie-Aranga have been selected in a 15-strong squad to tour Australia early next month.
Emily Close and Claire Kersten have been named in the reserves.
The strong representation of Otago players comes as no surprise to New Zealand under-21 assistant coach Janine Southby.
Southby, a former coach of the Otago Rebels and coach of the Dunedin premier club side University-Albion, said Otago was on the cusp of a special era with many talented netballers in the region.
"I don't know whether it goes in cycles or not, but you look back 10 years ago and we had a lot of players come through, with the likes of the Jo Morrisons, Anna Rowberrys and Belinda Collings," she said.
"I think we might be at the start of another cycle."
Southby believes the University of Otago and Academy of Sport play a crucial role in attracting top players.
The challenge was to keep them in Otago after they finished their studies.
Otago last week won the under-21 national title in Hamilton, and earlier this year Otago won the Lois Muir Challenge, a competition set up to operate as a feeder tournament for the ANZ Championship.
One of the new players coming through is defender Jaimee Power, who has had a fortnight to remember.
She was named player of the tournament at the under-21 nationals and yesterday morning received news of her inclusion in the New Zealand under-21 squad.
The 18-year-old University of Otago student was excited when she spoke to the Otago Daily Times yesterday: "I got a phone call from Janine and the emotions were just everywhere," she said.
Power made the New Zealand under-21 training squad last year and she was full of enthusiasm.
"Long-term, I'd like to make the [Silver] Ferns, but in the next couple of years I'd like to make the under-21 world cup team," she said.
