About 20 minutes into the second half, two players competing for the ball clashed heads before hitting the ground.
Queenstown player Elliott Gardner got to his feet quickly after the incident, but Roslyn-Wakari's Tom Bealin remained on the ground as supporters rushed to his side.
Bealin remained conscious, covered in blankets, as onlookers waited for ambulance personnel to arrive.
Once tended to by St John paramedics, Bealin was able to get up and walk to an ambulance with assistance.
The sight of him walking off the field was one of relief for Roslyn-Wakari coach Colin Thom.
``At the end of the day, football doesn't matter too much. It's more about the welfare of our players,'' Thom said.
``He is all right and that is the main thing.''
The match, the closest of contests, resumed shortly after Bealin left the ground following a 25-minute break in play.
The teams had contested an intense first half during which neither attack could get through the opposing defence.
The Queenstown side had the bigger share of possession and territory in the half, but its shots were all wide of the goal.
Less than 10 minutes into the second half the deadlock was broken through some good Queenstown Rovers link play.
Striker Carlos Hermann put his body on the line to score. He was winded in the process but was back on the field only minutes later.
As the sun started to go down on Ellis Park, so did the home side's chances of a win.
Though Roslyn-Wakari fought bravely to try to equalise, it simply could not find the back of the net and Rovers ran out 1-0 winners.
Queenstown coach Jamie Whitmarsh felt the result was a fair indication of the game.
``I thought we defended well and we got what we deserved in the end,'' he said.
``I know it is only round two, but when you get an away game it's going to be tough and they beat us 4-2 down here recently, so we had a point to prove.''
Whitmarsh credited a brave Roslyn-Wakari team for rallying after the injury to Bealin. Coach Thom was pleased with his team's effort under the most trying circumstances.
``It's disappointing to finish the way we did and still lose the game. They got a good goal just after halftime, but our boys dug deep.''
Dunedin Technical was the big winner of the second round of the Chatham Cup, running out a 6-0 winner over Timaru side West End.
The other two games of the round were closer. Green Island downed Northern 3-1 and Caversham beat Otago University 4-0.
Caversham's match was moved to Bayfield Park due to water lying on the pitch at Tonga Park.
There were two contrasting games in the women's Knockout Cup competition over the long weekend.
Roslyn-Wakari and Otago University were goalless after an epic duel during regular time, but Roslyn sealed the a 2-0 win with two goals in extra time.
There was certainly no extra time needed for Dunedin Technical, as it beat Queenstown 9-1.
The draws for the next rounds of the Chatham Cup and the Knockout Cup are expected to be completed this week.
