In April divers found one mature Undaria plant and several juvenile plants on the line of the wreck of the boat Stella, which was lying on its side on the shore of Chalky Inlet.
Environment Southland biosecurity and biodiversity operations manager Ali Meade said they would continue to control the marine pest within the area.
''We are absolutely gutted to find more Undaria in Fiordland. However, it's very important that we don't give up now as there's still a chance to control it.
''We continue to urge vessel operators to be vigilant.
''When you come into Fiordland your hull and gear must be clean.''