The choir consists of 22 children who have been orphaned, most often by Aids.
They have been given a home in the Ugandan village of Watato with other children and a house mother by the charity Watato Child Care Ministries.
Accompanied by nine adults, the choir performs on behalf of Tear Fund New Zealand in the Stanley St church at 7pm, the second date of its five-date tour of the South.
The choir will perform its "lively and evangelical songs" in more than 30 venues around the South Island within a month.
Tear Fund concert organiser Jane Laurie, of Invercargill, said she was excited it was the South Island's turn to enjoy the "joyful singing, the vibrant costumes and infectious smiles" of the Ugandan orphans.
Ms Laurie said the choir usually toured the North Island every year, "so this is a huge opportunity for South Islanders to hear these delightful and courageous children".
"The last time they toured the South Island was in 2007."
The Watoto choir and the holistic care programme have become well known around the world.
The choir has performed for Queen Elizabeth at Buckingham Palace and for former United States president George W. Bush.
About 2500 patrons packed the Dunedin Town Hall during the choir's last South Island tour.
