Invasion Day events kicked off in major capital cities and regional centres with organisers saying January 26, the anniversary of the arrival of the First Fleet in 1788, is not a day to celebrate.
In Melbourne, tens of thousands lined the steps of state parliament, in what organisers described as the biggest turnout to date, to demand justice for Indigenous deaths in custody and missing and murdered women, and holding governments to account in treaty negotiations.
Chants of "shame", "long live the resistance" and "from the river to the sea, always was always will be" rang out throughout the crowd as a dozen speakers addressed the crowd.
Indigenous activist and historian Dr Gary Foley said the failed voice referendum was proof the greatest obstacle to a better future was "Anglo-Australian racism born out of fear and ignorance" of history.
"We need to gently educate those who are not here today about the true nature of Australian history and why it is every year we gather here on this occasion," he told the crowd.
Pro-Palestine activists who usually rally in central Melbourne each Sunday joined the protest, with demonstrators shutting down Russell and Swanston Streets as they chanted and marched towards Flinders Street Station.
Democracy in Colour national director and Palestinian woman Noura Mansour said the plight of Indigenous Australians and Palestinians were intertwined.
"We were never meant to survive ... but the fact that we are here over 200 years later, demanding treaty now, demanding land back, calling for a free Palestine is a miracle," she told the cheering crowd.
Aboriginal and Palestinian flags were brandished while banners read, "Australia: Stolen land, Palestine: Stolen land" and "237 years, yet no treaty".
Amid a large police presence in the city, the demonstration was largely peaceful with a few minor scuffles between protesters and members of the public.
Wiradjuri woman Lily Hodgson said she was at the rally for her ancestors.
"When you look into my eyes you'll see my ancestors," she told AAP.
"I'm here for everything that was taken from us, in our language, in our spirituality, and in our connection the country."
The rally headed towards Redfern, with thousands peacefully marching through the streets of Sydney's CBD amid a heavy police presence surrounding the protesters, including police horses.
Victoria Police said they had done "extensive planning" for Australia Day events and rallies while NSW Police promised to use "proactive policing strategies" to ensure minimal impact on the community.
Speaker Uncle Bill criticised the Queensland government for scrapping the state's truth telling inquiry as one of the Liberal National Party's first moves in power.
"Things for Indigenous people are going down the drain," he told the crowd.
The crowd marched across Brisbane River to Musgrave Park chanting "always was, always will be Aboriginal land" as police officers lined the road.
Activists in Hobart are also marching to state parliament while in ACT an event was to take place at the Aboriginal Tent Embassy.
Other protests and cultural events were also being held in Perth, Adelaide and Darwin.