Ben Smith and Nasi Manu will lead the team as co-captains.
The squad, announced this morning, is made up of 32 players on full contract and also five players in the wider training group.
Twenty three players are returning from the 2013 season.
Kurt Baker returns to the Highlanders after a season with the Crusaders, and he is joined in the backs by Malakai Fekitoa (Auckland), Patrick Osborne (Canterbury), Bay of Plenty captain Willie Ripia, and Winston Stanley (Force).
In the forwards Tasman captain Shane Christie joins the southern franchise, alongside Pumas International Matias Diaz, Kane Hames (Bay of Plenty) and Otago players Tom Franklin and Gareth Evans.
The wider training group includes two Otago players in Lee Allan and Craig Miller, and also Richard Buckman (Hawke's Bay), Trent Renata (Waikato) and Ged Robinson (Hawke's Bay).
Joseph said the squad is well balanced and has depth across the board.
Joseph also added that selecting Ben and Nasi was reflective of the approach the team would be taking to the 2014 Super Rugby season.
"As a coaching group, we've selected two players we know will lead from the front and who have the experience and respect of the whole squad."
The Highlanders begin their season with a home match at Forsyth Barr Stadium on Saturday 22 February against the Blues.
Forwards
Josh Bekhuis, Shane Christie, Liam Coltman, Matias Diaz, Elliot Dixon, Gareth Evans, Ma'afu Fia, Tom Franklin, Kane Hames, John Hardie, Jarrad Hoeata, TJ Ioane, Chris King, Nasi Manu, Brayden Mitchell, Brad Thorn, Joe Wheeler.
Backs
Kurt Baker, Phil Burleigh, Jason Emery, Malakai Fekitoa, Patrick Osborne, Hayden Parker, Buxton Popoalii, Willie Ripia, Aaron Smith, Ben Smith, Lima Sopoaga, Winston Stanley, Fumiaki Tanaka, Shaun Treeby, Frae Wilson.
Wider training group
Lee Allan, Richard Buckman, Craig Millar, Trent Renata and Ged Robinson