Richard Trainor of Good Company Tours and Cobb & Co's Christine Strathern say the closure will affect their businesses.
The closure was announced last Saturday.
The section of road from Anzac Ave to the entrance to the Dunedin Railway Station will be made into a pedestrian mall, restricting almost all vehicles from the strip in front of the historic building, independent commissioner Louise Taylor decided.
Any challenge to the decision needs to be made to the Environment Court within 30 working days of the announcement.
Mr Trainor, whose company made submissions to a hearing on the issue before the decision was made, said he was considering appealing.
He said trials undertaken by the Dunedin City Council had not been done on days when two large cruise ships were at Port Chalmers, when bus parking was at its most difficult.
New coach and bus parking would be created in Anzac Ave and Castle St to counter those lost outside the railway station, but on busy cruise ship days he had already seen buses double-parked all around the area.
Parking away from the station entrance, as would be required, would make it difficult for passengers with mobility problems, something very common among bus tour passengers, and there needed to be ways to deal with that.
"I'm not 100% against it; there just need to be better solutions."
Ms Strathern, general manager of Cobb & Co, said she was still considering whether to appeal.
The company argued in its submission it also needed direct access for elderly and disabled customers to its restaurant at the railway station, something necessary for its financial stability.
She was keeping communications open with the council, and hoped to find a solution that would continue access to its door.
The effect of the road closure on the business was uncertain, but it was the sort of change that made business owners nervous, she said.
