Tareq Kamleh, who lived in Cumberland Hall during 2003 and studied first-year health sciences at Otago, surfaced in another Isis propaganda video posted to YouTube recently.
In it, the former South Australian paediatric registrar, who assumed the name Abu Yousef al-Australie and rose to prominence after fronting a propaganda video for the terrorist organisation in 2015, issued a call to arms.
He also hit out at fellow Muslims living in the West.
"It really surprises me how the Muslim nation is acting at the moment, how stagnant you have all become," Kamleh, who appears with a rifle and heavy facial hair in a tunnel, said in the video.
"You have no honour, you have no self-respect, you have no love for fellow Muslims."
It was the first time Kamleh had surfaced since appearing in the 2015 video.
Staff at the US embassy in Canberra would not confirm whether Kamleh was on the FBI’s terrorist screening database.
"The US Government does not provide information as to who is on the watchlist," a spokesman said.
It is estimated about 1 million people are on the FBI’s terrorist watchlist.
Australian police were granted an arrest warrant for Kamleh in 2015 on charges of being a member of a terrorist organisation, recruiting for a terrorist organisation and being in a declared area, likely the IS stronghold of Raqqa.
The latest video is believed to have been recorded in the Syrian city of Raqqa.
Kamleh left Australia for Syria in 2015.
He was known as Tex during his time in Dunedin and was a part of the university’s drinking scene and popular with women.
He failed to gain entry into the university’s medical degree programme and returned to Australia.