The popular Alexandra ground has been a regular feature on the domestic schedule since 1979 and had been pencilled in for two one-day matches in December.
But those matches will now be played in Queenstown and Dunedin after the pitch was deemed to be below the required standard.
The ground lost its New Zealand Cricket (NZC) warrant of fitness at the end of last season and failed to measure up during a recent trial match.
Molyneux Park Trust administrator Mark Tait told the Otago Daily Times he was saddened, both personally and for the community, but said it was a sign of the times.
"NZC are demanding higher standards in terms of the pitch and the facilities," Tait said.
"Their turf manager Jared Carter went through what I think was a fair process about evaluating the pitch and, unfortunately, it did not come up to scratch this time.
"There were issues with the way the pitch played in general and . . . the board is working through what we need to do to get it right."
Asked if the trust had confidence in the groundsman, Richard McAlevey, Tait replied: "I can't comment on that yet. We've had a board of trustees meeting and there are some steps to happen after that, but it is at a very delicate stage."
McAlevey refused to comment when contacted by the ODT.
While Otago Cricket Association chief executive Ross Dykes expressed disappointment, he remained optimistic the Volts would return to Molyneux Park.
"It is a great disappointment for everybody but it is certainly not the end of the world," he said.
"The Molyneux Park Trust, NZC and Otago cricket and Otago country cricket are all committed to getting that warrant restored as soon as possible.
"We are hopeful of doing the required work on the ground so another trial could potentially be held before the end of this summer."
Otago's December 8 one-day match against Wellington will shift to the Queenstown Events Centre, and the December 28 game against Central will be moved to the University Oval.
Otago's match against Northern Districts on December 20 may also have to shift if Oamaru's Centennial Park does not come up to standard.
The venue does not have a NZC warrant of fitness and will need to prove its suitability before the match can be confirmed.
Dykes said Otago cricket was looking to hold a trial at the ground in early January so the pitch and venue could be assessed.