Funds from the opportunity shop, in Centennial Ave, will be used to help finance the operations of the Otago SPCA.
The shop opened on Monday, and had a queue of customers waiting by the time the door was unlocked at 10am. Business was brisk with a steady stream of sales.
Alexandra-based SPCA inspector and fundraiser Helen Saunders said the shop was a first for the organisation in Otago.
It would be staffed by volunteers and goods were donated by the public, but the shop would have to "pay its own way" by generating enough income to cover rent and power, she said.
Stock includes used clothing, from formal gowns through to work gear, books, art works, "bric-a-brac", jewellery and SPCA merchandise.
Mrs Saunders said the shop would not affect the Salvation Army's Alexandra shop as research had shown having more than one opportunity shop in a town brought in more outside customers.