Mr O'Brien - better known as Mr Yipadee - has launched a fundraising album from his home in Manchester, England, to raise money for young Syrians fleeing their homeland.
O'Brien already boasted two albums that have topped the UK children's charts, and his latest effort, Sing-along for Syria, has tapped into ''a galaxy of stars''.
That included Coronation Street star Tina O'Brien, other UK children's TV stars, and New Zealand's Craig Smith, better known as the Wonky Donkey Man.
The album was released on iTunes last week, and O'Brien said all money raised would go to Save the Children's Child Refugee Crisis Appeal.
O'Brien said he had been left ''devastated'' while watching the plight of young Syrian refugees, just weeks after the birth of his second child.
''As a parent, I can't just sit by and watch what is happening. We wouldn't allow this to happen to our children. Why would we sit by and watch while the Syrian children suffer?"
The idea for the album came in the middle of the night, and within 48 hours O'Brien had used his contacts to recruit more than a dozen TV presenters to the project.
Smith said he, too, had been moved as a parent to join the effort.
''With the birth of my daughter earlier this year, how could I stand by and watch without getting involved?
''I hope that this album can spread joy to the parents and kids that buy it and help with needed resources to the kids and parents of Syria.''
O'Brien has lived in the UK for the past 13 years but grew up in Mosgiel, attending St Mary's School and the Taieri High School.
He most recently returned to Mosgiel in 2013 to visit his parents and his old school.
At the time, he said his Mr Yipadee character was developed to promote positive thinking and self-belief in children.
His career change came in 2012, after he lost his job at a Manchester radio station, burnt through his savings and had to borrow money for food.
The turning point came when fan mail arrived from Men are from Mars, Women are from Venus author John Gray.
A month later, Mr Yipadee's first album topped the United Kingdom iTunes children's chart, and within weeks he was headlining the children's concert at the Glastonbury Music Festival.
''That was incredible. There was the Rolling Stones and all these amazing bands playing and here was little old Mr Yipadee.''
